Participants completed electronic computer-based or a paper based questionnaire that collect data on lifestyle and health factors. Questionnaires collected information on socio-demographic and socio-economic status, current and past occupation (based on CNO-11), on lifestyle behaviors: history of tobacco (short version of Fagerstrom test) and alcohol consumption (WHO classification), physical activity (short version EPIC PAQ), dietary habits (14-item Mediterranean Diet Adherence Screener, FFQ), on medical history (participants, parents, siblings, children), medical history (including brief Mental Health Inventory, asthma score), prescription drug use (ATC classification), and women and men’s health.

The geolocalisation of residences was performed to evaluate environmental exposures (information from Urban Atlas, Corine Land Cover, Strategic Noise Map, Landsat and ISS images).

Physical measures were done: weight, height, waist and hip circumference, systolic and diastolic blood pressures, and heart rate.

A blood sample was taken from each participant (blood plasma, blood serum, white blood cells).

Molecular profile was determined for 6500 participants (6400 unrelated and 50 family trios). These 6400 unrelated participants were randomly selected from the cohort with a 1:1 gender proportion. General metabolic characterisation and specific lipoprotein profiles of 5000 blood plasma samples are determined. from the 6400 unrelated participants, 5459 genomic profiles have been characterised by genotyping. The role of DNA methylation on chronic disease heritability is evaluated on 50 related participants (parents and at least one offspring). The entire genome of 808 participants will also be sequenced; 200 with overlapping array and sequencing characterisation will be further analysed for somatic genetic variance in hereditary cancer genes.