Lifestyle, socio-economic factors, physical activity, use of tobacco and alcohol, medications and previous illnesses were assessed through questionnaires. Women were also asked about reproductive history. Information on diet were collected and dietary measurements where performed. Anthropometric measurements were taken, fat percent was calculated, carotid ultrasound and artery stiffness examinations were also taken. Blood samples were collected and DNA has been extracted. Further, B-mode carotid ultrasound examination to determine intima-media thickness and plaque on the carotid artery and laboratory tests were performed on the participants who agreed to participate in the cardiovascular project. Information about health and disease events has been obtained by sinchronization with administrative databases.

Data about physical activity, education, smoking habits, alcohol consumption, dietary habits, state of health, the use of drugs and nutritional supplements and diseases in the family were assessed through the questionnaire. Additional information until end of follow-up was obtained from the Southern Swedish Regional Tumour Registry, which provides up-to-date information on cancer incidence. Information on vital status was retrieved up to 31 December 1999 from the National Tax Board.

Data on B-mode carotid ultrasound examination to determine intima-media thickness and plaque on the carotid artery and laboratory tests were performed on the participants who agreed to participate in the cardiovascular project.